CPSC 265: Genetic Engineering Laboratory

This laboratory course provides a hands-on introduction to the techniques and principles of genetic engineering, recombinant DNA and the impact of molecular genetics and genomics on society. Students will isolate DNA from plants and clone specific genes into bacterial plasmids, perform polymerase chain reactions, DNA restriction analysis, RNA extraction, and analysis of genetically engineered plants. Discussions center on the relevance and use of these techniques to both agricultural and medical biotechnology.

Texts and Lab fee: There is no required text. There is a laboratory fee of $25 which is due on the first day of class. Laboratory protocols will be handed out.

Outline of topics:
Section I: Getting started (safety, lab equipment, pipeting)
Section II: Extract and analyze DNA and RNA
Section III: Detect specific genes and their expression in complex organisms
Section IV: Cloning a gene using polymerase chain reaction (PCR) and sequence analysis
Section V: Genetic engineering of plants and animals

By the end of this course, you will:

• Gain hands-on laboratory experience including the following:

• Extract and analyze DNA & RNA from bacteria and eukaryotes -Perform polymerase chain reaction (PCR) to amplify DNA

• Clone genes into bacteria, analyze transformed plants

• Discuss the relevance of recombinant DNA, cloning, and genomics, to medicine, agriculture, and society.

• Gain good laboratory experience that is useful for independent study projects

Kris N. Lambert
N-423 Turner Hall
Phone: (217) 265-5165
E-mail: knlamber@uiuc.edu (preferred method of contact)

Grades are NOT based on the plus/minus system:

• A (90-100)
• B (80-89)
• C (70-79)
• D (60-69)
• F (<60)