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Crop Sciences

College of Agricultural, Consumer, and Environmental Sciences
University of Illinois at Urbana-Champaign

CPSC 566: Plant Gene Regulation (cont.)

4 hours

Outline of Topics Used in Spring 2000
Transposable element influence on plant gene expression and variation
History and overview

Jan 19 Wed - Elements in bacteria and other species

Jan 21 Fri - Discovery of mobile elements in maize

Jan 24 Mon - Genetic behavior of transposable elements

Jan 26 Wed - Molecular methods review and update

Molecular isolation and
characterization of element families

Jan 28 Fri - The maize element activator (Ac)

Jan 31 Mon - The maize element suppressor-mutator (Spm)

Feb 2 Wed - Tam elements in snapdragon, elements in other species

Feb 4 Fri - Mutator sequences in maize

Feb 7 Mon - Retroviral-like elements

Transposable element action

Feb 9 Wed - Effects on gene expression

Feb 11 Fri - Effects on gene expression, continued

Feb 14 Mon - Role in evolution and diversity

Feb 16 Wed - Origin and regulation

Feb 16 Wed - Written critique is due

Understanding gene
regulation methods

Feb 18 Fri - Isolating regulatory genes whose products are unknown by gene tagging with transposable elements

Feb 21 Mon - First test (through Feb 16 material)

Feb 23 Wed - Molecular methods: review of transformation methods, Agrobacterium tumefaciens and DNA gun

Feb 25 Fri - Use of transposable elements in heterologous plant systems to isolate genes and study gene action

Selected examples of
regulatory genes

Feb 28 Mon - Anthocyanin pathway regulatory genes as models

Mar 1 Wed - Anthocyanin pathway regulatory genes, cont.

Identifying sequences that
regulate gene expression

Mar 3 Fri - Cis acting regions that regulate gene expression

Mar 6 Mon - Detection of DNA binding proteins

Mar 8 Wed - Transacting factors that regulate gene expression

Mar 10 Fri - Post transcriptional regulation, chloroplast gene regulation

  March 11-19 Spring Break
Sense and antisense effects on
gene expression

Mar 20 Mon - Antisense RNA technology to study gene expression

Mar 22 Wed - Co-suppression phenenomena are discovered first in plants

Mar 24 Fri - Co suppression effects in transgenic plants and multigene families

Seed proteins as models for
gene expression, subcellular
localization, and modification

Mar 27 Mon - Overview of seed storage proteins in monocots and dicots

Mar 29 Wed - Maize zein proteins and genes, control of zein synthesis; opaque-2 as a model system, transcriptional activators

Mar 31 Fri - Seed protein genes in legumes - glycinins, conglycinins, trypsin inhibitors, and lectins, synthesis and processing

Apr 3 Mon - Developmental and tissue specific regulation, mutations

Apr 5 Wed - Protein targeting reviews

Apr 7 Fri - Targeting and subcellular localization

Apr 10 Mon - Modification of proteins in transgenic plants / approaches and problems

Apr 12 Wed - Second test (on material from Feb 18 to Apr 10)

Plant functional genomics
arrives on the scene
Apr 14 Fri - Overview of technologies and goals in genomics: EST libraries, global gene expression, microarrays
Student led presentations/discussions
on recent topics in plant gene
regulation/genomics
Apr 17 Mon
Apr 19 Wed
Apr 21 Fri
Apr 24 Mon
Apr 26 Wed
Apr 28 Fri
May 1 Mon
May 3 Wed